Producing a mammalian gfp expression vector containing. Thermo scientific fermentas fastdigest enzymes are an advanced line of restriction enzymes for rapid dna digestion. William smith general phone 2755731 molecular biology sales specialist. Thermo scientific instruments, equipment, software, services and consumables empower scientists to solve for complex analytical challenges in pharmaceutical, biotechnology, academic, government, environmental and industrial research, as well as the clinical laboratory. During our 30 years of restriction enzyme research, we compiled one of the largest collections of restriction enzyme producing bacterial strains in the industry. Doubledigest conveniently calculates the best enzymatic reaction buffer, enzyme concentrations, incubation conditions, and any additives needed in your double digest reaction. Doubledigest calculatorthermo scientific thermo fisher. Thermo scientific a commitment to analytical precision. Any robust ethical theory will have prescriptive or normative statements that are not just actionguiding, but actionmotivating as well. Such bacteria can spread across the production lines of the food industry or drinking water.
Producing a mammalian gfp expression vector containing neomycin resistance gene. Thermo scientific fastdigest enzymes product information. Thermo scientific fermentas fastdigest restriction enzymes. The classification is according to the latest editions of the eulists, and extended by company and literature data. A lysozyme and magnetic bead based method of separating. The universal buffer allows rapid single, double, or multiple dna digestion within 515 minutes eliminating any need for buffer change or subsequent dna cleanup steps. To eliminate the background recircularisation, vectors with compatible ends were dephosphorylated. One unit is defined as the amount of noti required to digest 1 g ptz19rjl2 dnabseli fragments in 1 hour at 37c in 50 l of recommended reaction buffer.
Construction of a new minicircle dna carrying an enhanced. U of rochester biological supply ctr g8633 stock manager. To ask other readers questions about miraclw miracle of theismplease sign up. Having supplied restriction enzymes to the research community for over 40 years, neb has earned the reputation of being the leader in enzyme technologies.
1364 1039 360 1105 1180 188 1553 897 1356 1169 1116 1349 761 569 552 1364 1265 1539 860 592 868 1239 1025 1179 76 997 1411 305 1134 1076 197 193 666 1051 1082 888 157 306 215 202